ABSTRACT
<p><b>OBJECTIVE</b>To find a convenient and exact method for evaluating acrosome reaction in human spermatozoa.</p><p><b>METHODS</b>The semen of the normal male was mixed and then divided into 6 groups. Coomassie brilliant blue (CBB) staining, chlortetracycline (CTC) fluorescence staining and acid phosphatase (ACP) detection were used for morphological observation and data analysis of the acrosome status of the human sperm treated with or without progesterone.</p><p><b>RESULTS</b>There were obvious morphological differences between the acrosome-reaction and acrosome-intact spermatozoa in CBB staining and CTC fluorescence staining, and significant differences were observed between the experimental and control spermatozoa by the three methods (P < 0.05).</p><p><b>CONCLUSION</b>All the three methods can be used to assess acrosome reaction in human spermatozoa, but Coomassie brilliant blue (CBB) staining is much more convenient and stable.</p>